Part:BBa_K734002:Experience

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Applications of BBa_K734002

Lambert_GA 2022

Lambert iGEM attempted to characterize Spinach to validate the improvements of iSpinach-D5-G30-A32 (BBa_K4245000) and iSpinach (BBa_K3380150) . iSpinach is a mutant of Spinach developed by researchers at the University of Strasbourg. Compared to Spinach, iSpinach is less salt-sensitive, has higher thermal stability, and produces more fluorescence (Autour et. al, 2016). iSpinach-D5-G30-A32 is a co-crystallized, re-engineered version of iSpinach developed by researchers at the University of Strasbourg. We created BBa_K4245001, a composite part producing Spinach; BBa_K4245002 , a composite part producing iSpinach; and BBa_K4245003 , a composite part producing iSpinach-D5-G30-A32. The only difference is the basic aptamer part, allowing us to accurately compare the aptamers. However, after failed attempts at cloning, Lambert iGEM decided to continue the characterization of the various Spinach aptamers in 2023.

Reference:

Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083

User Reviews

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